Cell Lab

Cell Lab Materials and Methods and Results for lab 4. Materials and Methods Homogenization Spinach leaves were used. The bountiful veins were outback(a) and the sample was weighed. Four grams were used to start. The leaves were chopped fine and added to an glass-cold howitzer with 15 ml of homogenization buffer( .33M sorbitol, .05M HEPES, 4mM MgCl2, 2mM EDTA, pH 6.5). The leaves were grinded to a spreading and filtered through two layers of cheesecloth. The filtrate was collected, and severally lab collection was given a 1.5 ml fractional of the filtrate. We took a 100uL of this firmness of purpose and put into a microfuge underground labelled ?C?. Centrifugation The microfuge tube was set deflexion on ice while the remain filtrate was centrifuged at 200g?s. The supernatant was removed and 100ul were set aside in a tube designate ?LSS? (for low zip up supernatant) on ice. The rem aining supernatant was centrifuged at 1000g?s for 7 minutes. 100 uL were taken from supernatant, and placed in a...If you want to unsex a full essay, order it on our website: BestEssayCheap.com

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